Analysis of DNA fragmentation in bovine somatic nuclear transfer embryos using TUNEL
نویسندگان
چکیده
منابع مشابه
Analysis of DNA fragmentation in bovine somatic nuclear transfer embryos using TUNEL.
The production of cloned animals is an inefficient process because of early or late embryonic losses. This study focused on the DNA fragmentation that occurs during embryonic development. The occurrence of DNA fragmentation was examined in bovine embryos produced by in vitro fertilization (IVF) and somatic cell nuclear transfer (NT) using the terminal deoxynucleotidyl transferase (TdT) nick-end...
متن کاملTransgenic Bovine Nuclear Transfer Embryos from Adult Somatic Cell Lines*
This study was performed to examine whether adult fibroblast cells can be manipulated genetically and whether clonal lines derived from those cells can support embryonic development. A primary adult cell line was established from the ear of an aged cow. Adult cells with a plasmid containing the enhancer green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning. Gre...
متن کاملMitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ˙OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), ...
متن کاملMicrotubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type
This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic cells were fused to enucleated oocytes treated with either 5 mM caffeine or 150 microM roscovitine to control the type of nuclear remodeling. The proportion of embryos that under...
متن کاملGeneration of bovine transgenics using somatic cell nuclear transfer
The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have either been inefficient or not available in all animals, bovine included. More recently somatic cell nuclear transfer has provided a method to cre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction
سال: 2002
ISSN: 1470-1626,1741-7899
DOI: 10.1530/reprod/124.6.813